FIGURE 4.

ODAM induced RhoA signaling pathway in ameloblasts. A, ALCs were transfected with ODAM or ODAM siRNA constructs. RhoA signaling component expression was analyzed by Western blot. B, ALCs were transfected with ODAM, ODAM siRNA, ARHGEF5, or active RhoA constructs. Equal amounts of cell lysates were used for G-LISA RhoA activation assays. C, mapping the ODAM domain required for RhoA activation with ARHGEF5. FLAG-ODAM mutants were expressed in ALCs transfected with the ARHGEF5 construct. RhoA activity was determined by G-LISA RhoA activation assays. Data are mean ± S.D. of triplicate experiments. *, p < 0.05 compared with the control. D, FLAG-tagged ODAM and GFP-tagged RhoA constructs were transfected into ALCs. Exogenous ODAM was immunostained using anti-FLAG antibody, and GFP-RhoA was detected by immunofluorescence. Nuclei were stained with DAPI. Scale bars = 20 μm.