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. 2015 Apr 24;290(23):14740–14753. doi: 10.1074/jbc.M115.648022

FIGURE 5.

FIGURE 5.

ODAM induced actin rearrangement in ameloblasts via RhoA signaling. A, cells were cultured on rODAM- or collagen-coated slides for 24 h. Fixed cells were treated with rhodamine-phalloidin to examine actin filament rearrangement using confocal laser microscopy (red). ODAM localization was investigated by immunofluorescence. Scale bars = 10 μm. B, ALCs were treated with rODAM or transfected with active RhoA constructs. Equal amounts of cell lysates were used for the G-LISA RhoA activation assay. C, ALCs were transfected with ODAM, and rhodamine-phalloidin was used to examine the arrangement of actin filaments (red). Scale bars = 10 μm. D, ODAM, ODAM siRNA, or active RhoA constructs were transfected into ALCs. ALCs were treated with ROCK inhibitor (Y-27632). Rhodamine-phalloidin was used to examine the arrangement of actin filaments (red). ODAM localization was investigated by immunofluorescence. Scale bars = 20 μm. E, adhesion of ALCs to ODAM- or collagen-coated slides. Binding values are on the basis of the absorbance of adherent cells. Data are presented as mean ± S.D. of triplicate experiments. *, p < 0.05 compared with the control.