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. 2015 Apr 28;290(23):14754–14764. doi: 10.1074/jbc.M115.642124

FIGURE 4.

FIGURE 4.

The I/R-responsive induction of EST was Nrf2-dependent. A and B, primary mouse hepatocytes were exposed to 6-h hypoxia followed by 12-h reoxygenation. Nrf2 protein expression was measured by Western blotting, with the quantification shown in the right panel (A). The mRNA expression of Nrf2 target genes was measured by real-time PCR (B). *, p < 0.05; **, p < 0.01; all compared with the normoxia groups. C and D, primary mouse hepatocytes were treated with indicated doses of butylated hydroxyanisole (BHA) for the indicated amounts of time. The mRNA expression of EST (C) and Nrf2 target genes (D) was measured by real-time PCR. *, p < 0.05; **, p < 0.01; all compared with the vehicle group. E and F, female WT and Nrf2−/− mice were subjected to 60-min/12-h liver I/R. The EST mRNA (E) and protein (F) levels were measured by real-time PCR and Western blotting, respectively. n = 4–6. *, p < 0.05; NS, statistically not significant.