FIGURE 6.

Nrf2 ablation protected female mice from I/R liver injury. A–C, WT and Nrf2^−/− male mice were subjected to 60-min ischemia and 12-h reperfusion. Liver injury was measured by H&E staining of the liver paraffin sections with the necrotic areas (N) circled (A) and serum ALT and AST activities (B). The expression of the Nrf2 target gene was measured by real-time PCR (C). D–F, experiments were the same as described in A–C except that WT and Nrf2^−/− female mice were used. n = 4–6; *, p < 0.05; **, p < 0.01; NS, statistically not significant.