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. 2015 Apr 13;290(21):13028–13038. doi: 10.1074/jbc.M114.630558

FIGURE 1.

FIGURE 1.

The NanoSight detection system accurately quantifies ACVs from chondrocyte-conditioned medium. A, medium aliquots were tested in the NanoSight before (“unconditioned”) and after (“conditioned”) exposure to chondrocytes. Representative curves show typical results. B, conditioned medium were removed from chondrocyte cultures and counted in the NanoSight without further preparation (“media-derived”) or after centrifugation at 2000 × g for 15 min and then at 130,000 g for 60 min. The resultant pellet was resuspended in DMEM and counted in the NanoSight (“pellet-derived”). Values represent means ± S.D. There were no differences in the numbers of ACVs counted in the resuspended pellet and un-centrifuged medium (n = 3, p > 0.05). C, ACVs were counted in conditioned medium from chondrocytes plated at 4 × 105/cm2 or 2 × 105/cm 2. The numbers of ACVs correlated with the number of plated cells (n = 3, ***, p < 0.001). D, consistent increase in numbers of ACVs are identified under similar culture conditions, as shown by the scatter plot of ACV number at various time points.