Skip to main content
. 2015 Apr 13;290(21):13028–13038. doi: 10.1074/jbc.M114.630558

FIGURE 7.

FIGURE 7.

The effect of rapamycin on ACV number is dependent on caspase-3 but not mimicked by apoptosis agonists. A, porcine chondrocytes were exposed to rapamycin for 2 h and then either no additional additives or 50 μm of the caspase inhibitor Z-VAD-FMK for 2 h. ACVs were enumerated. Values represent means ± standard deviations. Z-VAD-FMK reduced the effect of rapamycin on ACV number (n = 3, *, p < 0.05) (B) without altering levels of LC3-II. C, porcine chondrocytes were incubated with doxorubicin or staurosporine for 4–12 h. ACVs were counted in conditioned medium. Values represent means ± S.D. Neither doxorubicin nor staurosporine affected ACV number (n = 9, p > 0.05). D, porcine chondrocytes were incubated with doxorubicin or staurosporine for 4–12 h with and without rapamycin. ACVs were counted in conditioned medium. Neither doxorubicin nor staurosporine increased ACV number (n = 9). E, porcine chondrocytes were incubated with doxorubicin or staurosporine for 6 h. Caspase-3 levels were measured to assess apoptosis (according to manufacturer's instructions). Both agents increased caspase levels in comparison to control (n = 3, p < 0.05).