FIGURE 8.

Rho/ROCK-dependent cell-blebbing is necessary but not sufficient for ACV release. A, cytoplasmic extensions are visible by EM in porcine chondrocytes at 12 h after rapapmycin treatment. B, porcine chondrocytes were incubated with or without the Rho/ROCK inhibitor, 10 μm Y27632 for 4–12 h in the presence and absence of rapamycin. ACVs were counted in conditioned medium. Values represent means ± S.D. Y27632 suppressed the effect of rapamycin on ACV number (n = 9, *, p < 0.05; **, p < 0.01). C, porcine chondrocytes were incubated with or without 100 μm or 1 mm ATP. ACVs were counted in conditioned medium after 4–12 h. Values represent means ± S.D. ATP had no effect on ACV number (n = 9, p > 0.05).