FIGURE 5.

HIRA in regulation of definitive hematopoiesis. A–D, HE cells and EBs were generated from control and Hira^−/− ES cells, cross-linked with 1% formaldehyde, and subjected to ChIP. The plots represent the recruitment of RUNX1 at the enhancer rich regions of Pu.1 and Gfi1 and at the enhancer elements (CNE3 and CNE2) of Gfi1b (means ± S.E. for three independent experiments). Preimmune rabbit serum (PI) is used as the negative control. Statistical analyses were performed using Student's t test function. *, p < 0.05; **, p < 0.01; ***, p < 0.001.