MS-MS analysis of the main monomer muropeptides from wild-type and ΔltsA C. glutamicum strains.
A, fragmentation of a reduced disaccharide tetrapeptide containing an amidated DAP residue. This muropeptide was isolated from wild-type C. glutamicum strain ATCC 13032 (peak C in Fig. 4). Panel a, fragmentation of the ion at m/z 940.6; panel b, inferred structure. B, fragmentation of a reduced disaccharide tetrapeptide containing a nonamidated DAP residue. This muropeptide was isolated from ΔltsA C. glutamicum strain (peak 2 in Fig. 4). Panel a, fragmentation of the ion at m/z 941.6; panel b, inferred structure.