. 2015 Apr 6;290(21):13079–13094. doi: 10.1074/jbc.M115.642843

TABLE 2.

Effect of the expression of LtsA_Cg on DAP amidation and cross-linking of the peptidoglycan in C. glutamicum and E. coli cells

	C. glutamicum ATCC 13032	C. glutamicum ΔltsA	E. coli	E. coli + LtsA_Cg
% of DAP_NH2 in monomers^a	80%	Not detected	Not detected	70%
% of DAP_NH2	80%	Not detected	Not detected	45%
In dimers^a	41% (1 DAP_NH2)			26% (no DAP_NH2)
	59% (2 DAP_NH2)			57% (1 DAP_NH2)
				17% (2 DAP_NH2)
Cross-linking^b (%)	32.5%	35%	29%	22%

^a Relative abundances of muropeptides were calculated from peak areas, as described previously by Glauner (34).

^b Overall peptide cross-linking (% dimers/% monomers + 2 × % dimers) is shown (34).