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. 2015 Apr 1;290(21):13104–13114. doi: 10.1074/jbc.M114.597922

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — GTP dependence of LHCP insertion into thylakoid membranes of Chlamydomonas. Thylakoids isolated from Chlamydomonas were incubated with in vitro translated, radiolabeled mature Cr-LHCP (Cr-mLHCP) (A) or precursor Cr-LHCP (Cr-pLHCP) (B) in the presence or absence of a stromal extract from Chlamydomonas and GTP or ATP as indicated. After incubation, thylakoids were protease-treated and washed with NaOH to remove non-integrated Cr-LHCP. Cr-LHCP-DP indicates a degradation product of precursor Cr-LHCP (Cr-pLHCP) generated by thermolysin treatment of thylakoids after the insertion reaction. The asterisk (*) indicates an unspecific background translation product.