TABLE 1.
Interaction of various Chlamydomonas and Arabidopsis cpSRP54 M constructs with Arabidopsis cpSRP43
The yeast strain Y190 was cotransformed with the indicated combinations of bait and prey constructs. In the Cr-54M/At-54C-term fusion construct, the C terminus of Chlamydomonas cpSRP54 M was replaced with that of Arabidopsis containing the cpSRP43-binding motif. The growth of the transformants on selective medium (−LTH) and β-galactosidase activity was analyzed.
| Constructs (bait) | At-43 (prey) |
|
|---|---|---|
| −LTH | β-Gal | |
| Cr-54M | − | − |
| Cr-54M/At-54C-term | ++ | + |
| At-54M(A530M) | ++ | + |
| At-54M(A530V) | ++ | + |
| At-54M(T534M) | ++ | + |
| At-54M(A535M) | − | − |
| At-54M(A535V) | + | − |
| Cr-54M(V529A) | ++ | + |