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Interaction of various Chlamydomonas cpSRP43 constructs with Arabidopsis cpSRP54M
The yeast strain Y190 was cotransformed with the indicated combinations of bait and prey constructs. The growth of the transformants on selective medium (−LTH) and β-galactosidase activity was analyzed.
| Constructs (prey) | At-54M (bait) |
|
|---|---|---|
| −LTH | β-Gal | |
| Cr-43 | − | − |
| Cr-43(D251E,E252Y,F276W) | − | − |
| Cr-43(P255V) | − | − |
| Cr-43(D251E,E252Y,P255V,F276W) | + | + |
