FIGURE 5.

The impact of pharmacological inhibitors or protein expression and viability in HT-GFP and HT-CerS6 cells. A, Western blot analysis of cells treated with 5 or 10 μm SP600125 and 25 μm curcumin for 28 h. B, viability in the presence of SP600125 or curcumin was measured over 3 days and results expressed as a percentage of untreated cells. Data shown are the average ± S.D. from three experiments. C, cell viability in the presence of LCL-521 after 24 h of treatment. A representative experiment performed in triplicate is shown. Similar results were obtained in two additional analyses.