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. 2015 Mar 31;290(21):13168–13177. doi: 10.1074/jbc.M114.631754

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Cell surface expression of HS on HEK293 cells overexpressing EXTL2. A–C, EXTL2-overexpressing, mock-transfected, and wild-type cells were examined for cell surface expression of HS by flow cytometry using the 10E4 antibody (A) and the 3G10 antibody before (B) and after (C) heparitinase (Hep) digestion. The histograms show the intensity of the fluorescence (percentage of maximum (% of max)) and is representative of two independent experiments. Black line, EXTL2-overexpressing cells; dashed line, mock-transfected cells; dotted line, wild-type cells; filled gray profile, control (secondary antibody only). PE-A, Phycoerythrin-Area.