. 2015 Mar 31;290(21):13168–13177. doi: 10.1074/jbc.M114.631754

TABLE 3.

Disaccharide composition of labeled HS isolated from mock or EXTL2 transfected HEK 293 cells.

Labeled samples were degraded by deamination at pH 1.5, followed by reduction of products with NaBH₄. The resulting ³⁵S- or ³H-labeled disaccharides were analyzed by anion-exchange HPLC (see “Experimental Procedures”). The values are given from single analyses or as means from metabolic labeling of two different EXTL2-overexpressing clones or mock-transfected clones ± mean deviation.

Cell type	³⁵S-labeled deamination products^a				³H-labeled deamination products^a
Cell type	GlcA-aMan_R6S^b	IdoA-aMan_R6S	IdoA2S-aMan_R	IdoA2S-aMan_R6S	GlcA-aMan_R6S	IdoA-aMan_R6S	IdoA2S-aMan_R	IdoA2S-aMan_R6S
	%				%
HEK 293^c	ND^d	ND	ND	ND	16	6	57	21
Mock	15 ± 0	6.5 ± 0.5	56.5 ± 3.5	22 ± 4	17.5 ± 0.5	6.5 ± 0.5	44.5 ± 1.5	31 ± 3
EXTL2	17.5 ± 2.5	7 ± 1	60.5 ± 1.5	16 ± 6	16 ± 0	5.5 ± 0.5	55 ± 2	23.5 ± 2.5

^a % of total O-sulfated disaccharides.

^b Untransfected 293 cells.

^c aMan_R, the 2,5- anhydromannitol deamination products of GlcNS residues.

^d ND, not determined.