Quantitative RT-PCR |
|
|
MyoD
|
AGC ACT ACA GTG GCG |
CAC TAT GCT GGA CAG |
Myogenin |
AGG CGC TGT GGG AGT |
CAA CCA GGA GGA GCG |
Myh3
|
GCC AGG ATG GGA AAG |
GGG CTC GTT CAG GTG |
P21
|
CCC TCT ATT TTG GAG |
GTA CCC TGC ATA TAC |
H2A.Z
|
GAG TTG GCA GGA AAT |
GAT GAC ACC ACC ACC |
Enhanced GFP |
GCA GAA GAA CGG CAT |
ACG AAC TCC AGC AGG |
HPRT
|
GCC TAA GAT GAG CGC |
TAC TAG GCA GAT GGC |
|
ChIP-quantitative PCR |
|
|
MyoD promoter (PRR) |
CGC CCC CAG CCT CCC TTT CCA G |
TGT CAG AGG AGT GGT GAA GAA A |
MyoD upstream |
GGA TGG GGC TCT CAA TGT CAG CG |
TGA GTG TGC GTG CCT TCA CCA |
Myogenin promoter |
GAA TCA CAT GTA ATC CAC TGG A |
ACA CCA ACT GCT GGG TGC CA |
Myogenin upstream |
GCC CAT CAC AGT TAG GAG CGG C |
ACT GTG TTT CTC GGC AAC CCC A |
CER |
GGG ACA GCC GCC TCC AAA CG |
TGA AGC GCC GGA CTC CAG GAA |
−10kb |
ACC CAG GGC ACC CCA AGT GT |
CGG GCT GTC TTA GGG GAA GCC T |
DRR |
ATG GCG GCA GGA GAA CTG AGC |
CAG CCA CCC CTT CTG GAG CG |
β-Actin promoter |
GCT TCT TTG CAG CTC CTT CGT TG |
TTT GCA CAT GCC GGA GCC GTT GT |
|
ChART-PCR |
|
|
MyoD PRR |
TAG ACA CTG GAG AGG CTT GGG CAG |
CTG GGC TAT TTA TCC AGG GTA GCC |
MyoD −7kb |
GGC ATG GGA GGT TTA TAG CA |
ATG CCA CTA TGC AAT CCA CA |
GAPDH −1.5kb |
GAC TCT GAA TCT GCC ATG CCT C |
CCA GAG CCA AGG CTG TGT TAG |