FIGURE 3.
Kinetic flux profiling by mass spectrometry reveals glucose-induced but not glucocorticoid-induced changes in metabolites associated with stimulus-secretion coupling. 832/13 cells were exposed to either DMSO or 10 nm Dex overnight. The cell culture medium was then replaced with a Hepes balanced salt solution containing either 3 or 15 mm [U-13C]glucose, and cells were harvested at the time points indicated. Cellular extracts were analyzed by UPLC-MS for 13C incorporation into water-soluble metabolites. Replicate data are shown as means ± S.D.