FIGURE 6.

MS6 displays both transactivation and transrepression activity in promoter-luciferase reporter assays. 832/13 cells were transfected with luciferase reporter constructs containing either −3.6 kb of the Ccl2 gene promoter (CCL2) or three copies of a consensus GRE in tandem (3xGRE). A, C, E, and G, 24 h post-transfection, cells were pretreated for 1 h with the indicated concentrations of Dex (A), HC (C), MS4 (E), and MS6 (G) and then cultured for 4 h with 1 ng/ml IL-1β. B, D, F, and H, cells were treated for 4 h with the log₁₀ of the steroid concentrations as shown on the x axis. Data are means ± S.E. (error bars) from 3–4 individual experiments. ***, p < 0.001 versus DMSO (black bar); **, p < 0.01 versus DMSO (black bar); *, p < 0.05 versus DMSO (black bar).