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. 2015 Apr 7;290(21):13401–13416. doi: 10.1074/jbc.M114.632190

FIGURE 7.

FIGURE 7.

Mercaptosteroids suppress Ccl2 and Ccl20 gene expression and secretion of protein. A–H, 832/13 cells were pretreated with the indicated concentrations of steroids for 1 h followed by a 6-h exposure to 1 ng/ml IL-1β. Relative mRNA abundance of Ccl2 (A, C, and E) and Ccl20 (G) was normalized to RPS9. ***, p < 0.001 versus DMSO (G); **, p < 0.01 versus DMSO (A and E); *, p < 0.05 versus DMSO (A, C, and E). CCL2 (B, D, and F) and CCL20 (H) release into the medium was measured by ELISA and normalized to total protein content. ***, p < 0.001 versus DMSO (black bar; G); **, p < 0.01 versus DMSO (black bar; B, D, and F); *, p < 0.05 versus DMSO (black bar; B and D); #, p < 0.1 versus DMSO (black bar; F). Data are expressed as means ± S.E. (error bars) from three independent experiments.