FIGURE 9.

MS6, but not MS4, promotes adipocyte differentiation. A, primary rat hepatocytes were treated with 100 nm Dex, MS4, or MS6 in the presence 10 μm forskolin (FSN). Following RNA isolation, Pck1 (Pepck) mRNA abundance was measured and normalized to RPS9. B, murine 3T3-L1 preadipocytes were induced to differentiate using the standard MDI induction mixture containing 100 nm Dex (CTL) or 100 nm each of either MS4 or MS6. Cell monolayers were subjected to Oil Red O staining 5 days after the induction of adipogenesis. RNA was isolated from the conditions indicated in B, followed by detection of transcript levels of Pref1 (C), adiponectin (D), Fabp4 (E), and Pparg (F), followed by normalization to the housekeeping gene RPS9. Data are shown as means + S.E. (error bars) for two independent experiments, each performed in duplicate. ***, p < 0.001; **, p < 0.01; *, p < 0.05.