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Proposed secondary structures of the pre-tRNA substrates used in this study. Both substrates are labeled at the 5′ end with fluorescein (Fl) and contain 5-nt leaders and a discriminator base at the 3′ end. The cleavage sites are identified with an arrow. A, A. thaliana mitochondrial pre-tRNACys. B, B. subtilis pre-tRNAAsp.
