. 2015 Mar 27;290(21):13454–13464. doi: 10.1074/jbc.M115.644831

TABLE 1.

Kinetic parameters for PRORP1-catalyzed A. thaliana pre-tRNA and B. subtilis pre-tRNA cleavage

Reactions were measured at 25 °C in 30 mm MOPS, pH 7.8, 150 mm NaCl, 1 mm TCEP, and 1 mm MgCl₂ as described in the legend of Fig. 3. Values reported reflect the mean of two independent experiments with the error reported as the standard deviation.

	Multiple turnover^a			Single turnover,^b k_max
Pre-tRNA	K_m	k_cat	k_cat/K_m	Single turnover,^b k_max
	nm	s⁻¹	m⁻¹ s⁻¹	s⁻¹
A. thaliana	630 ± 60	0.06 ± 0.01	9.8 ± 1.0 × 10⁴	0.04 ± 0.003
B. subtilis	350 ± 50	0.03 ± 0.003	1.0 ± 0.2 × 10⁵	0.03 ± 0.003

^a Measured using the FP assay with 20 nm PRORP and 0.06–3 μm pre-tRNA.

^b Measured using a stopped assay with 5 μm PRORP1 and 20 nm pre-tRNA.