FIGURE 5.
Vps2p helix 5 peptide C stimulates Vps4p ATPase activity and stabilizes the hexamer. A, ATPase activity of Vps4p was measured in the presence of different concentrations of peptide C. Normalization of ATPase activity was carried out with respect to wild-type Vps4p. B, oligomerization of Vps4pΔMIT-Vta1pVSL (10 μm Vps4p subunits, 1.7 μm hexamers) was measured by gel filtration in the presence of ADP·AlFx and in the presence (blue) or absence (gray) of 50 μm peptide C. Error bars indicate S.D. from three independent experiments.