TABLE 1.
Bacterial expression vectors
All expression plasmids have been deposited at the DNASU Plasmid Repository at Arizona State University (71).
| Construct | Insert | Backbone | Internal ID |
|---|---|---|---|
| Vps4pΔMIT | Vps4p (81–437) | pET151-D-Topo | CPH2473 |
| Vps4pΔMIT E243A | Vps4p (81–437, E243A) | pET151-D-Topo | CPH2675 |
| Vps4pΔMIT E247A | Vps4p (81–437, E247A) | pET151-D-Topo | CPH2678 |
| Vps4pΔMIT E243A, E247A | Vps4p (81–437, E243A,E247A) | pET151-D-Topo | CPH2779 |
| Vps4pΔMIT T240A | Vps4p (81–437, T240A) | pET151-D-Topo | CPH2673 |
| Vps4pΔMIT T240K | Vps4p (81–437, T240K) | pET151-D-Topo | CPH2783 |
| Vps4pΔMIT T240V | Vps4p (81–437, T240V) | pET151-D-Topo | CPH2803 |
| Vps4pΔMIT T240F | Vps4p (81–437, T240F) | pET151-D-Topo | CPH2784 |
| Vps4pΔMIT R241A | Vps4p (81–437, R241A) | pET151-D-Topo | CPH2575 |
| Vps4pΔMIT R251A | Vps4p (81–437, R251A) | pET151-D-Topo | CPH2576 |
| Vps4pΔMIT W206A | Vps4p (81–437, W206A) | pET151-D-Topo | CPH2574 |
| Vta1pVSL | Vta1p (280–330) | pET151-D-Topo | CPH2570 |
| MIM1-Vps4p | Vps2p(218–232)-(GGGGS)3-Vps4p | pET151-D-Topo | CPH2802 |
| MIM1(L225D)-Vps4p | Vps2p(218–232, L225D)-(GGGGS)3-Vps4p | pET151-D-Topo | CPH3093 |
| MIM2-Vps4p | Vfa1p(183–203)-(GGGGS)3-Vps4p | pET151-D-Topo | CPH3077 |
| MIM2(F198D)-Vps4p | Vfa1p(183–203, F198D)-(GGGGS)3-Vps4p | pET151-D-Topo | CPH3078 |
| GST | GST | pET151-D-Topo | CPH3094 |
| GST-peptide A | GST-Vps2p (165–201) | pET151-D-Topo | CPH2914 |