FIGURE 1.

Nuclear localization sequence of anillin lies in the first 91 amino acids. A, schematic of the domain organization of anillin and the GFP-anillin fusion proteins expressed in HeLa cells shown in B. FL, full-length anillin sequence; wt, wild type sequence. B, micrographs of HeLa cells transiently expressing different GFP-anillin fusion proteins described in A. C, quantitation of the ratio of GFP fluorescence between the nuclear and cytoplasmic compartments for HeLa cells expressing different GFP-anillin fusion proteins described in A and B. Bar, 5 μm. Myo, myosin; Act, actin; AH, anillin homology domain; PH, pleckstrin homology domain.