FIGURE 2.

N terminus of anillin has distinct conserved regions across different vertebrate species and binds to importin β2 in a Ran-dependent manner. A, alignment of the first 100 amino acids of anillin in different vertebrate species. B, consensus motifs for different classes of nuclear localization sequences. C, comparison of the anillin bPY motif to other bPY motifs. D, recombinant His₆-tagged importin α and β1 or β2 were incubated with a MBP-anillin(1–151) fusion protein. The MBP-anillin fusion protein was re-isolated on amylose beads, and co-purifying His₆-tagged proteins were detected by SDS-PAGE and Western blotting. E, His₆ importin β2-MBP-anillin binding reaction described in A was repeated in the presence and absence of recombinant GST-RanGTP. Alignment of the anillin importin β2-binding motif with other known importin β2-binding motifs.