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. 2015 Mar 31;290(21):13500–13509. doi: 10.1074/jbc.M115.649160

FIGURE 5.

FIGURE 5.

Importin β2-binding site on anillin does not overlap with the CD2AP- and mDia2-binding sites. A, recombinant MBP-anillin(1–151) was incubated with GST-mDia2(89–533) in the presence of increasing concentrations of His6-importin β2. MBP-anillin was re-isolated on amylose beads, and co-purified GST-mDia2 was detected by SDS-PAGE and Western blotting. B, recombinant MBP-anillin(1–151) was incubated with GST-CD2AP(1–350) in the presence of increasing concentrations of His6-importin β2. MBP-anillin was re-isolated on amylose beads, and co-purified GST-CD2AP was detected by SDS-PAGE and Western blotting.