FIGURE 6.

Importin β2 binding to anillin does not regulate anillin function during cytokinesis. A, GFP-anillin and GFP-mutant anillin stably expressing HeLa cells lines where GFP-anillin expression was under the control of the tetracycline repressor. Expression conditions were developed to induce GFP-anillin at near endogenous anillin levels. A siRNA strategy was developed to prevent the expression of endogenous anillin, although GFP-anillin expression continued. B, quantitation of the number of bi-nucleate cells observed upon expression of different GFP-anillins in the presence and absence of siRNA to deplete endogenous anillin protein levels. Error bars are ± S.E. C, micrographs showing nuclear morphology in HeLa cells expressing different GFP-anillins in the presence and absence of endogenous anillin. Bar, 5 μm.