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The sequences of the primer sets used for ChIP Seq
| Location of STAT6 relative to ATG | Sequence of primers | Annealing temperatures | PCR product sizes |
|---|---|---|---|
| Site 1 (−456) | F-ATAATGGGAAGGGGCTCTGG | 62 °C | 216 bp |
| R-CCCACTCACAATGCACACTC | |||
| Site 2 (−5694) | F-TTTGCTTATCTATGAAGTGGAGGT | 58 °C | 200 bp |
| R-CCAGATATTTGCCTTCGGTTTC | |||
| Site 3 (−8991) | F-AAGTGTGCATCGCCTTCA | 58 °C | 225 bp |
| R-TCCCTACACCTAGGATTAGAACA | |||
| Sites 4 and 5 (−9300 and −9334) | F-GGGCTGAAAGACCCATTAGATAG | 58 °C | 212 bp |
| R-GGTCTAGCTGTAATAATGGCAGAA |
