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MEP50 and PRMT5 are required for keratinocyte proliferation. A, KERn were electroporated twice with control, MEP50, or PRMT5 siRNA, and 15,000 cells/well were plated. After overnight attachment, the cell number was determined (day 0) and at the indicated times thereafter. The values are mean ± S.E. (n = 3). *, p < 0.005. B, immunoblot detection of MEP50 and PRMT5. The immunoblot confirms a reduction in MEP50 in MEP50 siRNA-treated cultures. PRMT5 is also reduced.
