Skip to main content
. 2015 Apr 13;290(21):13531–13540. doi: 10.1074/jbc.M115.636704

FIGURE 2.

FIGURE 2.

Transposase-DNA interactions in the crystal structure of the Mos1 IRL PEC and comparison with the IRR PEC structure. A, transposase residues His-65 to Arg-71, linking the HTH1 and HTH2 motifs, bind in the minor groove between bases 15–18 of the NTS. The linker is displaced out of the minor groove in the PEC IRL (green) as compared with the equivalent residues in the PEC IRR (orange). B, cis linker-DNA interactions involving the base pair G16:C41 in the PEC IRL (left panel). A stereo view is shown, and the 2Fo − Fc electron density map, contoured at 2σ, is displayed as a gray mesh. The right panel shows the equivalent interactions in the PEC IRR involving the T16:A41 base pair. C, interactions between HTH1 and the major groove in the inner region of the inverted repeat sequence. D, trans interactions between the catalytic domain and DNA involving the unpaired base G56 in the IRL PEC and A56 in the IRR PEC.