FIGURE 6.
KLF4 and KRT13 are required for SB-induced cell cycle arrest. A, KYSE450 cells transfected with KLF4 siRNA pools (si-KLF4) or nontargeting control (nc) treated or untreated with 4 mm SB for 48 h. Western blotting was performed to determine the expression of KLF4. β-Actin served as an internal control. B, KYSE450 cells transfected with KLF4 siRNA pools (siKLF4) or nontargeting control (nc) were seeded at 1.5 × 105 cells per well in conventional medium and treated or untreated (control) with 4 mm SB on 6-well plates; cells were stained with propidium iodide and analyzed by flow cytometry at 48 h. Bars represent the means ± S.D. C, KYSE450 cells were cotransfected FLAG-KRT13 or vector with KLF4 siRNA (siKLF4) or nontargeting control (nc) and treated with 4 mm SB for 48 h. Western blot analysis showed the expression of KLF4 and KRT13 in KYSE450 cells. β-Actin served as an internal control. D, cell viability at selected time points of each indicated treatment was analyzed by MTS cell proliferation array. Bars represent the means ± S.D. *, p < 0.05.