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. 2015 Apr 8;290(21):13578–13590. doi: 10.1074/jbc.M114.633339

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Sensorgrams showing the interactions between immobilized CohScaC to DocScaB (A) and DocI15S/N16I/I51S/N52I (B), monitored by SPR. CohScaC was immobilized on a CM5 sensor chip as described under “Experimental Procedures.” The sensor chip surface was subsequently exposed to different concentrations (shown in A) of wild-type Doc ScaB. The SPR sensorgrams are shown as dotted lines with kinetic fits of binding superimposed with solid lines. B, exemplifies a no binding result obtained with the DocI15S/N16I/I51S/N52I mutant. Both were fitted with a 1:1 Langmuir binding model using BIAevaluation software (Version 2.0.1). The affinity constant was calculated as k_off/k_on.