TABLE 1.

Primers used to obtain the genes encoding the cohesin and the dockerins used in this study

Engineered restriction sites are shown in bold and mutations are underlined.

Clone	Sequence (5′ → 3′)	Direction
DocScaB, DocI15S/N16I and DocI51S/N52I	CTCGAATTCAAGTTTATTTATGGCGATG	Forward
DocScaB, DocI15S/N16I and DocI51S/N52I	CACCTCGAGTAATTCCTTTTCTTCCACC	Reverse
DocI15S/N16I/I51S/N52I	GGCTCGGTTCGTTCGATTGATGCTGTGCTGATTCGTG	Forward
DocI15S/N16I/I51S/N52I	CACGAATCAGCACAGCATCAATCGAACGAACCGAGCC	Reverse
CohScaA	CACACCATGGCAAACGGGCTTTAATCTGAGC	Forward
CohScaA	CACACTCGAGATTGACAGCACCGTTGGTAAC	Reverse