FIG 1.

R6 inhibits the IFN response. (A) tRF-ISREs were transfected for 40 h with pRL-SV40 and either pcDNA3.1-R6HA or empty pcDNA3.1. The cells were then transfected with poly(I · C) and assayed at the indicated time points (bottom). Firefly luciferase levels were normalized to constitutively expressed Renilla luciferase levels in each well, and all samples were normalized to the positive control [empty vector plus poly(I · C) at 8 h post-poly(I · C) transfection]. The data are averages (and standard errors of the mean [SEM]) from 3 independent experiments and are represented as RLU. (B) Telomerized RFs were transfected with pcDNA3.1-R6HA or mock transfected for 40 h and subsequently transfected with poly(I · C) for 8 h. RNA was extracted and analyzed by RT-PCR. The values are relative to GAPDH levels. The data were analyzed using a paired t test. P values of less than 0.05 were considered significant, and values greater than 0.05 were not significant (NS).