FIG 6.

PK1 is a nucleocapsid component of both virions. (A) BVs and ODVs were purified from the supernatants and the pellets of vPK1:FLAG-infected cells, respectively, and fractionated into envelope (E) and nucleocapsid (NC) fractions. Immunoblotting was performed with anti-FLAG antibody to detect PK1, anti-ODV-E25 antibody to detect the BV/ODV envelope protein ODV-E25, anti-VP39 antibody to detect the major capsid protein VP39, and anti-GP64 antibody to detect the BV envelope-specific protein GP64. (B) Immunoelectron microscopy showing the distribution of PK1 in vPK1:FLAG-infected cells. At 60 h p.i., the cells were fixed, dehydrated, embedded in LR white resin, and processed for immunogold labeling with a mouse monoclonal anti-FLAG antibody. Shown are processing nucleocapsids in the VS (a), nucleocapsids of the mature ODVs (b), edge of the electron-dense matte regions of the VS (c). Open triangles point to the specific locations of 10-nm gold particles.