FIG 2.

Endogenous HCV replication in cell-free replication lysates is severely reduced in FBP1-kd cells but increased in FBP1-overexpressing MH14 cells. An aliquot of normalized replication lysates from MH14 cells in which FBP1 was either knocked down or overexpressed was examined for endogenous HCV replication activity; the radiolabeled RNA products were analyzed by denaturing agarose gel electrophoresis and visualized by autoradiography. Normalized cell-free replication lysates also were Western blotted (WB) for the expression level of FBP1, HCV NS5A, and actin. (Left) Lane 1, cured MH14 cells without HCV replicons; lane 2, control MH14 cells with HCV replicons; lane 3, FBP1-kd MH14 cells; lane 4, MH14 cells in which FBP1 was overexpressed. (Right) The percentage of endogenous replication products relative to those in controls.