FIG 9.

Colocalization of FBP1-p53 and FBP1-BCCIP and cellular distribution of p21 in control and FBP1-kd cells. Huh7.5 cells were grown on chamber slides and treated with goat anti-FBP1 antibody and Alexa 568-labeled secondary antibody (red), treated with mouse anti-p53 antibody (A) or mouse anti-BCCIP antibody (B), and then treated with Alexa 488-labeled secondary antibody (green). DAPI was used to stain the nuclei (blue). Cells were observed individually for FBP1, BCCIP, and p53 localization by a Nikon A1R confocal microscope. (C) The cellular distribution of p21 in control and FBP1-kd HepG2 cells. (Left) Distribution of p21 in the nuclear (N) and cytoplasmic (C) fractions of control and FBP1-kd HepG2 cells. Nuclear and cytoplasmic fractions from control and FBP1-kd cells were prepared and Western blotted for p21. PARP and tubulin also were Western blotted as specific markers for the nucleus and cytoplasm, respectively. (Right) Control and FBP-kd HepG2 cells were grown on a chamber slide for 24 h, fixed, and treated with anti-p21 antibody and Alexa 568-labeled secondary antibody (red). DAPI was used to stain the nuclei. The cellular distribution of p21 was observed under a confocal microscope.