TABLE 3.

ADAR1-mediated hypermutations in DI-RNA clones

Virus	Expt no. and plaque no.	DI clone	Polaritya	No. of hypermutations observed/no. of total nucleotidesb	% editingc
vac2-CKO	2.1	06/12	U → C	36/111	31.5
	2.3	08/03	U → C	6/141	4.3
	3.1	20/05	U → C	16/126	12.7
IC323-CKO	1.2	58/04	U → C	5/52	9.6
	1.3	59/03d	A → G	17/67	25.4
	2.2	60/06	U → C	8/21	38.1
	3.1	62/04d	U → C	19/54	35.2
	3.1	62/06d	A → G	28/67	41.8
	3.1	62/11d	U → C	11/54	20.4

^aPolarity indicates whether the hypermutations were observed as A → G or U → C in the genomic orientation.

^bThe number of mutations observed/number of total nucleotides of the respective type (A or U) in a cluster.

^cThe percentage is the calculated frequency of transitions observed over possible events.

^dThese sequences were derived through semispecific hybridization of primer A2 at position −15670 of the MV genome.