FIG 6.

Impact of DENV infection on degradation of p62. (A) Time course analysis of p62 protein amounts. Huh7 cells were mock inoculated or infected with DENV NGC (MOI = 5) for given periods. Samples were harvested, and 40 μg of total cell protein was subjected to SDS-PAGE and Western blotting with p62- and β-actin (loading control)-specific antisera. p62- and β-actin-specific signals were quantified by using the ImageJ software package. The bar graphs on the right represent the p62 amounts relative to those detected in mock-inoculated cells at the indicated time points. (B) Immunodetection of p62 in DENV-infected cells. Huh7 cells were either mock treated or infected with DENV NGC (MOI = 5) for 36 h. The cells were fixed with 4% PFA and stained for the viral NS3 protein and p62. Samples were analyzed by confocal microscopy imaging. The scatter blot on the right shows the total p62-specific signal/cell. Signals were quantified by using the ImageJ software package. The level of significance is denoted by asterisks (***, P ≤ 0.001).