TABLE 5.
Genes containing ORF-disrupting mutations in HCMV strains
| Gene | % of strains mutateda | Mutation type(s)b (no. of strains) | Median unaffected fraction of mutated ORFs (min–max) |
|---|---|---|---|
| UL9 | 34.6 (n = 127) | Sub in cod59 (5),c 23-nt del (4),c sub in cod6 (3),c 5-nt del (3),c sub in cod59 (2), 71-nt del (1),c sub in cod23 (1), sub in cod26 (1),c sub in cod40 (1),c sub in cod49 (1), sub in cod53 (1),c sub in cod59 (1), sub in cod63 (1), sub in cod71 (1),c sub in cod79 (1), sub in cod163 (1), 1-nt ins (1), 1-nt ins (1),c 1-nt ins (1), 178-nt del (1), 90-nt del (1), 51-nt del (1), 44-nt del (1), 40-nt del (1), 40-nt del (1), 29-nt del (1), 29-nt del (1),c 19-nt del (1), 19-nt del (1), 4-nt del (1), 2-nt del (1),c 1-nt del (1) | 27 (2–95) |
| RL5A | 20.3 | 11-nt del (14),c 2-nt del (4),c 17-nt del (3),c sub in cod12 (2),c sub in cod35 (1),c 44-nt del (1), 1-nt del (1) | 51 (6–58) |
| RL6 | 15.6 | 316-nt del (14),c 17-nt del (2), sub in cod57 (1),c 5-nt del (1), 2-nt del (1), 2-nt del (1) | 0 (0–66) |
| US9 | 15.0 (n = 127) | 35-nt del (18),c sub in cod227 (1)c | 94 (91–94) |
| UL1 | 10.2 | Sub in cod99 (6),c sub in cod86 (2), sub in cod147 (2), sub in cod42 (1), sub in cod45 (1),c 4-nt del (1) | 44 (8–68) |
| UL111A | 9.4 | Sub in cod36 (3),c 38-nt del (3),c sub in cod129 (2), sub in cod56 (1), 2-nt ins (1), 1-nt ins (1), 219-nt del (1)c | 32 (9–72) |
| UL150 | 6.3 | Sub in cod1 (1), 2-nt deletion (7)c | 1 (1–95) |
| UL40 | 5.5 | Sub in cod1 (7)c | 93 (93–93) |
| US7 | 5.5 (n = 127) | Sub in cod161 (1), sub in cod179 (1), 1-nt ins (1),c 112-nt del (1), 76-nt del (1),c 67-nt del (1), 47-nt del (1)c | 71 (3–93) |
| RL13 | 3.9 | Sub in cod150 (1), 2-nt ins (1), 279-nt del (1), 2-nt del (1), 1-nt del (1) | 50 (23–80) |
| UL128 | 2.4 (n = 126) | Sub in splice donor site (1), 1-nt ins (1), 14-nt del (1) | 32 (19–41) |
| UL133 | 2.3 | Sub in cod247 (1), 1-nt ins (1), 37-nt del (1) | 78 (61–97) |
| US13 | 2.3 | 24-nt del (3) | 97 (97–97) |
| RL12 | 1.6 | 5-nt del (1), 2-nt del (1) | 36 (27–45) |
| UL136 | 1.6 | Sub in cod210 (1), sub in cod227 (1) | 91 (87–94) |
| US27 | 1.6 | 18-nt del (1), 43-nt del (1)c | 97 (97–97) |
| RL1 | 0.8 | 84-nt del (1)c | 87 (87–87) |
| UL11 | 0.8 | 1-nt del (1) | 39 (39–39) |
| UL30 | 0.8 | 1-nt ins (1)c | 73 (73–73) |
| UL148 | 0.8 | Sub in cod104 (1) | 33 (33–33) |
| UL145 | 0.8 | 349-nt del (1) | 54 (54–54) |
| UL142 | 0.8 | 77-nt del (1)c | 73 (73–73) |
| UL150A | 0.8 | Sub in cod264 (1) | 97 (97–97) |
| IRS1 | 0.8 (n = 126) | Sub in cod836 (1) | 99 (99–99) |
| US6 | 0.8 (n = 127) | Sub in cod35 (1) | 19 (19–19) |
| US12 | 0.8 | 2-nt ins (1) | 44 (44–44) |
a
n = 128, unless stated otherwise (mutations that are certain to be artifacts of culture passage are omitted).
b
del, deletion; ins, insertion; sub, substitution; cod, codon (codon refers to the actual codon position in the specific strain that contains the mutation).
c
Mutations that were verified to be present in original clinical material (or strains sequenced directly from clinical material).