FIG 11.
Effect of β1 integrin on HSV-1 nuclear egress. (A) sh-Luc-HEp-2, sh-β1 integrin-HEp-2, and sh-β1 integrin/β1 integrin-HEp-2 cells were infected with wild-type HSV-1(F) at an MOI of 10 (107 PFU/ml), fixed at 24 h postinfection, embedded, sectioned, stained, and examined by transmission electron microscopy. Arrowheads indicate invagination structures containing primary enveloped virions. Nu, nucleus; Cyt, cytoplasm; NM, nuclear membrane. Bars, 500 nm. (B) sh-Luc-HEp-2, sh-β1 integrin-HEp-2, and sh-β1 integrin/β1 integrin-HEp-2 cells were infected with wild-type HSV-1(F) at an MOI of 10 (107 PFU/ml), fixed at 24 h postinfection, embedded, sectioned, stained, and examined by transmission electron microscopy as described for panel A. The percentage of enveloped perinuclear virions was determined.