FIG 15.
Effect of HSV-1 infection on αV and α5 integrins. (A) HEp-2 cells infected with wild-type HSV-1(F) or YK715 (MEF-CD98hc) at an MOI of 5 (3 × 107 PFU/ml) for 24 h were harvested, immunoprecipitated with anti-Myc antibody, and analyzed by immunoblotting with the indicated antibodies. (B and C) HEp-2 cells were mock infected or infected with wild-type HSV-1(F) at an MOI of 5 (5 × 106 PFU/ml), fixed at 24 h postinfection, permeabilized, stained with the indicated antibodies, and examined by confocal microscopy. Bars, 10 μm.