Skip to main content
. 2015 May 20;89(15):7799–7812. doi: 10.1128/JVI.00741-15

FIG 7.

FIG 7

Effect of CD98hc on HSV-1 nuclear egress. (A) sh-Luc-HEp-2, sh-CD98hc-HEp-2, and sh-CD98hc/CD98hc-HEp-2 cells were infected with wild-type HSV-1(F) at an MOI of 10 (107 PFU/ml), fixed at 24 h postinfection, embedded, sectioned, stained, and examined by transmission electron microscopy. Arrowheads indicate invagination structures containing primary enveloped virions. Nu, nucleus; Cyt, cytoplasm; Nm, nuclear membrane. Bars, 500 nm. (B) sh-Luc-HEp-2, sh-CD98hc-HEp-2, and sh-CD98hc/CD98hc-HEp-2 cells were infected with wild-type HSV-1(F) at an MOI of 10 (107 PFU/ml), fixed at 24 h postinfection, embedded, sectioned, stained, and examined by transmission electron microscopy as described for panel A. The percentage of enveloped perinuclear virions was determined.