FIG 3.

PPS treatment does not alter the kinetics of viral replication, as indicated by measurement of both infectious virus and viral RNA. Mice were infected s.c. with RRV or mock infected with diluent alone and then either treated daily i.p. with PPS or mock treated with PBS alone. At days 1, 2, 3, 7, and 10 p.i., serum and quadriceps and ankle tissues were harvested and homogenized and the viral load determined. (A) For infectious virus, viral load was determined by plaque assay on Vero cells. (B) For viral RNA, viral load was determined by qPCR with nsp3-specific primers, using a standard generated from serial dilutions of RRV T48 infectious plasmid. Each symbol represents the result for a single mouse, and the horizontal lines represent the median values. *, P < 0.05; two-way ANOVA with Bonferroni posttest for plaque assay and unpaired Mann-Whitney for PCR.