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Repeated low-dose infection elicits greater inflammasome responses in mice. Mice (n = 8 to 10) were killed on day 5 after the first infection. Lung homogenates and BALF were collected for inflammasome response analysis using an ELISA. (A) IL-18 levels in lung homogenates. (B) IL-18 levels in BALF. (C) IL-1β expression in lung homogenates. (D) IL-1β concentrations in BALF. Data are presented as means ± standard errors of the means. ***, P < 0.0001; n.s., no significance.
