TABLE 1.
Viruses used in this study
| Virus | Reporter tag | Mutation | No. of rats with visual circuit transmission/total no. of ratsc | No. of transport events in avian DRG sensory neurons |
|
|---|---|---|---|---|---|
| Retrograde | Anterograde | ||||
| PRV-GS4284a | UL25/mCherry | None | 5/5 | 288 | 38 |
| PRV-GS5469 | UL25/mCherry | ΔUS9b | 0/5 | 192 | 35 |
a
PRV-GS4284 was described previously (16).
b
A stop codon was inserted after the start ATG, and codons 2 to 72 were deleted, thus removing all internal in-frame ATG codons.
c
Following intravitreal injection of WT or mutant virus into the eyes of rats, the number of rats exhibiting virus fluorescence in retinorecipient regions of the brain 48 h.p.i. (either lateral geniculate nucleus or superior colliculus) was determined (19).