FIG 2.

Brain-PI(4,5)P₂ and DO-PI(4,5)P₂, but not DP-PI(4,5)P₂, promote stable binding of Gag to POPS-based GUVs. Binding of either PH-GFP (A to D), WT Gag-YFP (E to H), or delNC Gag-YFP (I to L) to GUVs containing no PI(4,5)P₂ (A, E, and I), brain-PI(4,5)P₂ (B, F, and J), DO-PI(4,5)P₂ (C, G, and K), or DP-PI(4,5)P₂ (D, H, and L) was examined in the context of POPC+POPS+chol GUVs, as in Fig. 1. Lipid ratios used were as follows: POPC:POPS:chol, 46.6:23.3:30 (A, E, and I); POPC:POPS:chol:PI(4,5)P₂, 40:20:30:10 (B to D, F, G, and J to L). Representative images from at least 3 independent experiments are shown. Fluorescence intensity profiles along the lines drawn to cross over opposite sides of GUVs (X to X′) are shown below images. PH-GFP and WT and delNC Gag-YFP were imaged using the same confocal microscopy settings, and their line profiles are shown on the same y axis scale. Bar, 5 μm.