TABLE 4.
Frequencies of original and mutated amino acids observed in SA13/JFH1Core-NS5Bb
| HCV protein | Amino acid positiona | Original |
Mutant |
||
|---|---|---|---|---|---|
| Amino acid | Frequency (%) | Amino acid | Frequency (%) | ||
| Core | 114 | R | 91 | W | <1 |
| Core | 187 | V | 48 | A | <1 |
| E1 | 235 | V | 8 | L | 2 |
| E2 | 385 | T | 96 | P | <1 |
| p7 | 782 | L | 43 | V | 4 |
| NS2 | 900 | Y | 16 | C | <1 |
| NS5A | 2034 | N | 5 | D | 3 |
| NS5A | 2238 | E | <1 | G | 99 |
| NS5A | 2252 | V | 24 | A | <1 |
| NS5A | 2266 | L | 1 | P | <1 |
| NS5A | 2340 | I | 3 | T | <1 |
| NS5B | 2500 | A | 99 | S | <1 |
| NS5B | 2841 | V | 19 | A | 81 |
a
Positions are numbered according to the H77 reference sequence (GenBank accession no. AF009606).
b
HCV sequence alignments (2008 Web Alignment) of individual HCV proteins were downloaded from the Los Alamos HCV sequence database (71). Amino acid frequency across genotypes at the given positions was determined using BioEdit Sequence Alignment Editor 7.2.5.